Winter, lying 14th after the dressage, fell heavily while clearing an obstacle during the cross country stage, and was pronounced dead on arrival at hospital.
Saturday was also marred by the death of a horse in the event for the second year running.
Liberal, a 15-year-old gelding ridden by British rider Tom Crisp, collaped and died after clearing a jump.
Last year, P'tite bombe, a 10-year-old ridden by French rider Emeric George, had to be destroyed after a fall during the cross country stage.
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